325 Maltotetraose alleviates chronic itch by inhibitting substance p-neurokinin 1 receptor pathway

The number of people suffering from symptoms sensitive skin has been growing. Their main pains are specified as dryness and chronic itch. Recent studies showed that Substance P (SP) Neurokinin 1 receptor (NK1R) on the C nerve fibers related to Focusing this pathway, we discovered maltotetraose (MTO), a type polysaccharide, alleviated itch skin. Single-center, open-label clinical trials formulations containing 1.0% MTO were conducted subjects who have with As result, score was improved after application formulations, but detailed mode action not determined. This study aims elucidate mechanism how works an anti-itch substance. Permeability into human evaluated using chromatography (HPLC), detected both stratum corneum tissues under where elongated exist. Interactions between MTO, SP NK1R measured isothermal titration calorimetry (ITC). Binding constant MTO-NK1R 10 times higher than MTO-SP SP-NK1R. Calcium ion influx, which is final cascade development, monitored overexpressed HEK293 cells fluorescent calcium indicator. Fluorescence increase observed addition, suppressed by pre-treatment approximately 50% in dose-dependent manner (p<0.01). These results indicate reaches target site binds NK1R, blocking binding suppress development. We multilaterally elucidated inhibits one pathways